You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
----- setting up ouput folder bin_classify and merging contigs from all bins... -----
Warning: bin_classify already exists.
----- aligning bin_classify/all_contigs.fa to /media/rmvcc/Vivekananda/NCBI_nt/ncbi_nt_db -----
----- database with MEGABLAST. This is the longest step - please be patient. You may look at the -----
----- classification progress in bin_classify/megablast_out.raw.tab -----
metawrap classify_bins -b /home/rmvcc/INITIAL_BINNING/BIN_REASSEMBLY/reassembled_bins/ -o bin_classify
metawrap classify_bins -b /home/rmvcc/INITIAL_BINNING/BIN_REASSEMBLY/reassembled_bins/ -o bin_classify
########################################################################################################################
ALIGN CONTIGS TO DATABASE WITH MEGABLAST
########################################################################################################################
----- setting up ouput folder bin_classify and merging contigs from all bins... -----
Warning: bin_classify already exists.
----- aligning bin_classify/all_contigs.fa to /media/rmvcc/Vivekananda/NCBI_nt/ncbi_nt_db -----
----- database with MEGABLAST. This is the longest step - please be patient. You may look at the -----
----- classification progress in bin_classify/megablast_out.raw.tab -----
/home/rmvcc/miniconda3/envs/metawrap-py27/bin/metawrap-modules/classify_bins.sh: line 126: 3444798 Segmentation fault (core dumped) blastn -task megablast -num_threads$threads -db $ {BLASTDB}/nt -outfmt '6 qseqid qstart qend qlen sseqid staxids sstart send bitscore evalue nident length' -query ${out}/all_contigs.fa > ${out}/megablast_out.raw.tab
***** Failed to run megablast. Exiting... *****
real 844m59.282s
user 599m56.630s
sys 17m36.518s
Even i try to run separately:
blastn -task megablast -num_threads 5 -db /media/rmvcc/Vivekananda/NCBI_nt/ncbi_nt_db/nt -outfmt 6 -query /media/rmvcc/Vivekananda/NCBI_nt/all_contigs.fa > output.tab
Segmentation fault (core dumped)
my system have 40 core 80 threads and 256 gb memory. Please help me.
The text was updated successfully, but these errors were encountered: